NET97 pmRFP-N2 (641)
(Plasmid
#61994)
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Purposemammalian expression of nuclear envelope transmembrane protein
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61994 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmRFP-N2
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Backbone manufacturerunknown
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAADACL1
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Alt nameNET97
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SpeciesH. sapiens (human)
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GenBank IDAAH28734.2
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Entrez GeneNCEH1 (a.k.a. AADACL1, NCEH)
- Promoter CMV
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Tag
/ Fusion Protein
- mRFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer mRFP1-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NET97 pmRFP-N2 (641) was a gift from Eric Schirmer (Addgene plasmid # 61994 ; http://n2t.net/addgene:61994 ; RRID:Addgene_61994) -
For your References section:
The leukocyte nuclear envelope proteome varies with cell activation and contains novel transmembrane proteins that affect genome architecture. Korfali N, Wilkie GS, Swanson SK, Srsen V, Batrakou DG, Fairley EA, Malik P, Zuleger N, Goncharevich A, de Las Heras J, Kelly DA, Kerr AR, Florens L, Schirmer EC. Mol Cell Proteomics. 2010 Dec;9(12):2571-85. doi: 10.1074/mcp.M110.002915. Epub 2010 Aug 6. 10.1074/mcp.M110.002915 PubMed 20693407