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Addgene

Emerin pEGFP-C1 (637)
(Plasmid #61993)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61993 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    unknown
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Emerin
  • Alt name
    EMD
  • Species
    H. sapiens (human)
  • Entrez Gene
    EMD (a.k.a. EDMD, LEMD5, STA)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer mRFP1-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Emerin pEGFP-C1 (637) was a gift from Eric Schirmer (Addgene plasmid # 61993 ; http://n2t.net/addgene:61993 ; RRID:Addgene_61993)

  • For your References section:

    System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics. Zuleger N, Kelly DA, Richardson AC, Kerr AR, Goldberg MW, Goryachev AB, Schirmer EC. J Cell Biol. 2011 Apr 4;193(1):109-23. doi: 10.1083/jcb.201009068. Epub 2011 Mar 28. 10.1083/jcb.201009068 PubMed 21444689
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