NET31 mRFP-N2 (490)
(Plasmid
#61984)
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Purposemammalian expression of nuclear envelope transmembrane protein
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61984 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N2
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNET31
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Alt nameTMEM209
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SpeciesH. sapiens (human)
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Entrez GeneTMEM209 (a.k.a. NET31)
- Promoter CMV
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Tag
/ Fusion Protein
- mRFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer mRFP1-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NET31 mRFP-N2 (490) was a gift from Eric Schirmer (Addgene plasmid # 61984 ; http://n2t.net/addgene:61984 ; RRID:Addgene_61984) -
For your References section:
NET23/STING promotes chromatin compaction from the nuclear envelope. Malik P, Zuleger N, de las Heras JI, Saiz-Ros N, Makarov AA, Lazou V, Meinke P, Waterfall M, Kelly DA, Schirmer EC. PLoS One. 2014 Nov 11;9(11):e111851. doi: 10.1371/journal.pone.0111851. eCollection 2014. PONE-D-14-28213 [pii] PubMed 25386906