pUC-dirTopo-P2A-aGFP
(Plasmid
#61974)
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PurposeA directional cloning vector of PCR-amplified cDNAs for mammalian expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61974 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namelacZ alpha
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SpeciesE. coli
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Insert Size (bp)132
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MutationIt contains silent mutations
Gene/Insert 2
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Gene/Insert nameEGFP
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SpeciesA. victoria
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Insert Size (bp)714
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC-dirTopo-P2A-aGFP was a gift from Hiroshi Udo (Addgene plasmid # 61974 ; http://n2t.net/addgene:61974 ; RRID:Addgene_61974)