pNAS1B split mCherry N-MESVD/TRAP(RK)-C
(Plasmid #61967)

• Purpose: Duet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVD
• Depositing Lab: Lynne Regan
• Publication: Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7. doi: 10.1021/cb500658w. Epub 2014 Oct 6.

Backbone

• Vector backbone: pNAS1B
• Backbone size w/o insert (bp): 3937
• Total vector size (bp): 5113
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Growth instructions: Please refer to Sawyer et al. (2014) ACS Chem. Biol. 9: 2502-7 for details about the required strains and growth conditions for performing the split mCherry assembly assay.
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: N-mCherry-MESVD
• Alt name: N-MESVD
• Insert Size (bp): 531
• Promoter: PLtetO

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: 5'-GATACTGAGCACATCAGCAGGACG-3'
• 3′ sequencing primer: 5'-GTAAGCCAGTATACACTCCGCTAGC-3'

Gene/Insert 2

• Gene/Insert name: TRAP(RK)-C-mCherry
• Alt name: TRAP(RK)-C
• Alt name: RK-C
• Promoter: PBAD

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: AatII (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: 5'-CCTACCTGACGCTTTTTATCGCAACTC-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry