pNAS1B split mCherry N-link/link-C
(Plasmid
#61965)
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PurposeNegative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61965 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNAS1B
- Backbone size w/o insert (bp) 3937
- Total vector size (bp) 4717
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Growth instructionsPlease refer to Sawyer et al. (2014) ACS Chem. Biol. 9: 2502-7 for details about the required strains and growth conditions for performing the split mCherry assembly assay.
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameN-mCherry-link
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Alt nameN-link
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Insert Size (bp)516
- Promoter PLtetO
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer 5'-GATACTGAGCACATCAGCAGGACG-3'
- 3′ sequencing primer 5'-GTAAGCCAGTATACACTCCGCTAGC-3' (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namelink-C-mCherry
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Alt namelink-C
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Insert Size (bp)264
- Promoter PBAD
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site AatII (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer 5'-CCTACCTGACGCTTTTTATCGCAACTC-3' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNAS1B split mCherry N-link/link-C was a gift from Lynne Regan (Addgene plasmid # 61965 ; http://n2t.net/addgene:61965 ; RRID:Addgene_61965) -
For your References section:
Designed Phosphoprotein Recognition in Escherichia coli. Sawyer N, Gassaway BM, Haimovich AD, Isaacs FJ, Rinehart J, Regan L. ACS Chem Biol. 2014 Nov 21;9(11):2502-7. doi: 10.1021/cb500658w. Epub 2014 Oct 6. 10.1021/cb500658w PubMed 25272187