4QPP
(Plasmid
#61964)
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PurposeBaculovirus expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61964 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFBOH-MHL
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Backbone manufacturerSGC (Addgene plasmid# 62304)
- Backbone size w/o insert (bp) 6767
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Vector typeBaculovirus Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsGentamicin (bacmid resistance in DH10Bac E. coli)
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHRMT1L6:PBC001-A12:C213642
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Alt namePDB IDs: 4QPP, 5E8R, 5HZM, 5WCF, 4QQK, 6P7I
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Alt nameHRMT1L6
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SpeciesH. sapiens (human)
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Insert Size (bp)1125
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MutationSee comments
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GenBank IDgi|8922514
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Entrez GenePRMT6 (a.k.a. HRMT1L6)
- Promoter Polyhedrin
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Tags
/ Fusion Proteins
- 6x His (N terminal on backbone)
- TEV cleavage site (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
- 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3') (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
4QPP was a gift from Cheryl Arrowsmith (Addgene plasmid # 61964 ; http://n2t.net/addgene:61964 ; RRID:Addgene_61964)