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Addgene

4QPP
(Plasmid #61964)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 61964 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFBOH-MHL
  • Backbone manufacturer
    SGC (Addgene plasmid# 62304)
  • Backbone size w/o insert (bp) 6767
  • Vector type
    Baculovirus Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Gentamicin (bacmid resistance in DH10Bac E. coli)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HRMT1L6:PBC001-A12:C213642
  • Alt name
    PDB IDs: 4QPP, 5E8R, 5HZM, 5WCF, 4QQK, 6P7I
  • Alt name
    HRMT1L6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1125
  • Mutation
    See comments
  • GenBank ID
    gi|8922514
  • Entrez Gene
    PRMT6 (a.k.a. HRMT1L6)
  • Promoter Polyhedrin
  • Tags / Fusion Proteins
    • 6x His (N terminal on backbone)
    • TEV cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    4QPP was a gift from Cheryl Arrowsmith (Addgene plasmid # 61964 ; http://n2t.net/addgene:61964 ; RRID:Addgene_61964)
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