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Addgene

4QPN
(Plasmid #61963)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 61963 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFBOH-MHL
  • Backbone manufacturer
    Structural Genomics Consortium
  • Vector type
    Baculovirus Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Gentamicin (bacmid resistance in DH10Bac E. coli)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    METTL21B
  • Alt name
    PDB:4QPN
  • Species
    H. sapiens (human); Plasmodium falciparum
  • Insert Size (bp)
    678
  • Mutation
    See comments
  • Entrez Gene
    METTL21B (a.k.a. FAM119B)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • MHHHHHHSSGRENLYFQG (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer Polyhedrin Forward (AAATGATAACCATCTCGC)
  • 3′ sequencing primer SV40 poly A Reverse
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    4QPN was a gift from Cheryl Arrowsmith (Addgene plasmid # 61963 ; http://n2t.net/addgene:61963 ; RRID:Addgene_61963)
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