4P7A
(Plasmid
#61954)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61954 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET28a-MHL
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Backbone manufacturerAddgene Plasmid 26096
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMLH1:JMC01L-F09:C205690
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Alt namePDB ID: 4P7A
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SpeciesH. sapiens (human)
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Insert Size (bp)1041
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MutationSee comments
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GenBank IDgi|28559089
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Entrez GeneMLH1 (a.k.a. COCA2, FCC2, HNPCC, HNPCC2, LYNCH2, MLH-1, MMRCS1, hMLH1)
- Promoter T7
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Tags
/ Fusion Proteins
- MHHHHHHSSGRENLYFQG (N terminal on backbone)
- 6xHis (C terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
4P7A was a gift from Cheryl Arrowsmith (Addgene plasmid # 61954 ; http://n2t.net/addgene:61954 ; RRID:Addgene_61954)