Skip to main content
Addgene

4P7A
(Plasmid #61954)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61954 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET28a-MHL
  • Backbone manufacturer
    Addgene Plasmid 26096
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MLH1:JMC01L-F09:C205690
  • Alt name
    PDB ID: 4P7A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1041
  • Mutation
    See comments
  • GenBank ID
    gi|28559089
  • Entrez Gene
    MLH1 (a.k.a. COCA2, FCC2, HNPCC, HNPCC2, LYNCH2, MLH-1, MMRCS1, hMLH1)
  • Promoter T7
  • Tags / Fusion Proteins
    • MHHHHHHSSGRENLYFQG (N terminal on backbone)
    • 6xHis (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    4P7A was a gift from Cheryl Arrowsmith (Addgene plasmid # 61954 ; http://n2t.net/addgene:61954 ; RRID:Addgene_61954)
Related items: