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Addgene

4QL1
(Plasmid #61942)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61942 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28-MHL
  • Backbone manufacturer
    SGC (Addgene Plasmid # 26096)
  • Backbone size w/o insert (bp) 7325
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WDR5:APC041-G05:C36415
  • Alt name
    PDB IDs: 4QL1, 5EAL, 5EAM, 5EAP, 5EAR
  • Species
    H. sapiens (human)
  • Mutation
    See comments
  • GenBank ID
    gi|61744459
  • Entrez Gene
    WDR5 (a.k.a. BIG-3, BIG3, CFAP89, SWD3)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6x His (N terminal on backbone)
    • TEV cleavage site (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    4QL1 was a gift from Cheryl Arrowsmith (Addgene plasmid # 61942 ; http://n2t.net/addgene:61942 ; RRID:Addgene_61942)
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