pDEST15.1-hRPN13 (285-386)
(Plasmid
#61936)
-
PurposeExpression of hRPN13 (285-386) in E.coli
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61936 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepDEST15
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7000
- Total vector size (bp) 7400
-
Modifications to backboneGSFT sequence after TEV site mutated to GSWT to aid in quantification by E280.
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namehRPN13 (285-386)
-
Alt nameADRM1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)400
-
MutationConstruct starts at residue 285, stop codon introduced after residue 386
-
GenBank IDNM_175573
-
Entrez GeneADRM1 (a.k.a. ARM-1, ARM1, GP110, PSMD16)
- Promoter T7
-
Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Stop codon introduced via Quickchange mutagenesis.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDEST15.1-hRPN13 (285-386) was a gift from Chris Hill (Addgene plasmid # 61936 ; http://n2t.net/addgene:61936 ; RRID:Addgene_61936) -
For your References section:
Structural Basis for the Activation and Inhibition of the UCH37 Deubiquitylase. VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson H, Cohen RE, Yao T, Hill CP. Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 10.1016/j.molcel.2015.01.016. 10.1016/j.molcel.2015.01.016 PubMed 25702872