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pET151-hUCH37 DM
(Plasmid #61931)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61931 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET151-D-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5800
  • Total vector size (bp) 6800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hUCH37 (ISF1) M269E/N291D
  • Alt name
    UCHL5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    990
  • Mutation
    Changed methionine 269 to glutamate and asparagine 291 to aspartate
  • GenBank ID
    AAD31528.1
  • Entrez Gene
    UCHL5 (a.k.a. CGI-70, INO80R, UCH-L5, UCH37)
  • Promoter T7
  • Tag / Fusion Protein
    • 6x HIS (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutations were incorporated via Quickchange mutagenesis.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET151-hUCH37 DM was a gift from Chris Hill (Addgene plasmid # 61931 ; http://n2t.net/addgene:61931 ; RRID:Addgene_61931)

  • For your References section:

    Structural Basis for the Activation and Inhibition of the UCH37 Deubiquitylase. VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson H, Cohen RE, Yao T, Hill CP. Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 10.1016/j.molcel.2015.01.016. 10.1016/j.molcel.2015.01.016 PubMed 25702872
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