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pET151-hUCH37 (ISF2)
(Plasmid #61930)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61930 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET151-D-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5800
  • Total vector size (bp) 6800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hUCH37 (ISF1)
  • Alt name
    UCHL5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    987
  • GenBank ID
    NP_001186190.1
  • Entrez Gene
    UCHL5 (a.k.a. CGI-70, INO80R, UCH-L5, UCH37)
  • Promoter T7
  • Tag / Fusion Protein
    • 6x HIS (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Isoform 2 was created by deletion of E246 by Quickchange mutagenesis from pET151-hUCH37 (ISF1) vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET151-hUCH37 (ISF2) was a gift from Chris Hill (Addgene plasmid # 61930 ; http://n2t.net/addgene:61930 ; RRID:Addgene_61930)

  • For your References section:

    Structural Basis for the Activation and Inhibition of the UCH37 Deubiquitylase. VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson H, Cohen RE, Yao T, Hill CP. Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 10.1016/j.molcel.2015.01.016. 10.1016/j.molcel.2015.01.016 PubMed 25702872
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