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PurposeExpression of S. cerevisiae URA3 cloned into pFA6a
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61924 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a
- Total vector size (bp) 3628
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Vector typeYeast genomic targeting
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameURA3
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SpeciesS. cerevisiae (budding yeast)
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GenBank IDAB540657.1
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The amplified region is functional, but does not overlap with the URA3 deletion region in BY4741 and derivatives, so will not recombine at the endogenous locus in this background.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a URA3 was a gift from Jon Houseley & David Tollervey (Addgene plasmid # 61924 ; http://n2t.net/addgene:61924 ; RRID:Addgene_61924) -
For your References section:
Repeat expansion in the budding yeast ribosomal DNA can occur independently of the canonical homologous recombination machinery. Houseley J, Tollervey D. Nucleic Acids Res. 2011 Nov 1;39(20):8778-91. doi: 10.1093/nar/gkr589. Epub 2011 Jul 17. 10.1093/nar/gkr589 PubMed 21768125
Map uploaded by the depositor.
