ML14
(Bacterial strain #61911)

• Purpose: C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. tyrA gene deleted.
• Depositing Labs: Robert Gennis, Toshio Iwasaki
• Publication: Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1016/j.ymeth.2011.08.019. Epub 2011 Sep 8.

Backbone

• Vector backbone: none

Growth in Bacteria

• Bacterial Resistance(s): None
• Growth Temperature: 37°C
• Growth Strain(s): ML14 (tyrA)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: none

Resource Information

• Supplemental Documents:
  • PCR primers
  • PCR data ML14

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Genotype = tyrA
Precursor strain = C43(DE3)
modified from the parent Escherichia coli C43(DE3) strain
Selective amino acid labeling (and/or requirement) = (Tyr)

Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system.

Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.