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Addgene

pAD01 S. cerevisiae telomerase RNA KBS
(Plasmid #61890)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61890 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Puc19
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 2758

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Telomerase RNA hairpin
  • Alt name
    TLC1 KBS
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    87
  • GenBank ID
    NC_001134.8
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

FokI site follows RNA construct to linearize plasmid prior to in vitro transcription.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAD01 S. cerevisiae telomerase RNA KBS was a gift from Thomas Cech (Addgene plasmid # 61890 ; http://n2t.net/addgene:61890 ; RRID:Addgene_61890)

  • For your References section:

    RNA recognition by the DNA end-binding Ku heterodimer. Dalby AB, Goodrich KJ, Pfingsten JS, Cech TR. RNA. 2013 Jun;19(6):841-51. doi: 10.1261/rna.038703.113. Epub 2013 Apr 22. 10.1261/rna.038703.113 PubMed 23610127
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