pGEX-6p-1 Sesn1b
(Plasmid
#61874)
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Purposebacterial expression of a GST-Sesn1b (mouse)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61874 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-6p-1
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 7479
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSesn1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1479
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Mutationnone
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Entrez GeneSesn1 (a.k.a. 1110002G11Rik, Pa26, Sest1)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-6p-1 Sesn1b was a gift from Ming Li (Addgene plasmid # 61874 ; http://n2t.net/addgene:61874 ; RRID:Addgene_61874) -
For your References section:
Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling. Peng M, Yin N, Li MO. Cell. 2014 Sep 25;159(1):122-33. doi: 10.1016/j.cell.2014.08.038. 10.1016/j.cell.2014.08.038 PubMed 25259925