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Addgene

pLJM1 Flag-Sesn2 419A/422A/426A
(Plasmid #61872)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61872 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLJM1
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 8440
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sesn2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1440
  • Mutation
    419A/422A/426A
  • Entrez Gene
    Sesn2 (a.k.a. HI95, SEST2, Ses2)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJM1 Flag-Sesn2 419A/422A/426A was a gift from Ming Li (Addgene plasmid # 61872 ; http://n2t.net/addgene:61872 ; RRID:Addgene_61872)

  • For your References section:

    Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling. Peng M, Yin N, Li MO. Cell. 2014 Sep 25;159(1):122-33. doi: 10.1016/j.cell.2014.08.038. 10.1016/j.cell.2014.08.038 PubMed 25259925
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