pLJM1 Flag-Sesn2
(Plasmid
#61871)
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PurposeLentivirial expression of Flag-Sesn2 (mouse)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61871 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLJM1
- Backbone size w/o insert (bp) 7000
- Total vector size (bp) 7440
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSesn2
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SpeciesM. musculus (mouse)
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Insert Size (bp)1440
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Mutationnone
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Entrez GeneSesn2 (a.k.a. HI95, SEST2, Ses2)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLJM1 Flag-Sesn2 was a gift from Ming Li (Addgene plasmid # 61871 ; http://n2t.net/addgene:61871 ; RRID:Addgene_61871) -
For your References section:
Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling. Peng M, Yin N, Li MO. Cell. 2014 Sep 25;159(1):122-33. doi: 10.1016/j.cell.2014.08.038. 10.1016/j.cell.2014.08.038 PubMed 25259925