pVenus-N1 (804)
(Plasmid
#61854)
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Purpose(Empty Backbone) empty backbone for tagging proteins at the C-terminus with Venus
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61854 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEYFP-N1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Tag
/ Fusion Protein
- Venus (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- Venus (C terminal on backbone)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Exactly the same as pEYFP-N1 but with AgeI-BsrGI swapped for venus fragment containing the point mutations F46L/F64L/V68L/M153T/V163A/S175G compared to EYFP
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVenus-N1 (804) was a gift from Jonathon Pines (Addgene plasmid # 61854 ; http://n2t.net/addgene:61854 ; RRID:Addgene_61854)