pEGFP-C2 Chfr (1014)
(Plasmid
#61853)
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Purposemammalian expresion of Chfr fused to EGFP
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61853 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C2
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChfr
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SpeciesH. sapiens (human)
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GenBank IDNM_001161344.1
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Entrez GeneCHFR (a.k.a. RNF116, RNF196)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-C2 Chfr (1014) was a gift from Jonathon Pines (Addgene plasmid # 61853 ; http://n2t.net/addgene:61853 ; RRID:Addgene_61853) -
For your References section:
Chfr acts with the p38 stress kinases to block entry to mitosis in mammalian cells. Matsusaka T, Pines J. J Cell Biol. 2004 Aug 16;166(4):507-16. Epub 2004 Aug 9. 10.1083/jcb.200401139 PubMed 15302856