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Addgene

pVenus-N1 cyclin B1 L45A (1324)
(Plasmid #61851)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61851 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pVenus-N1
  • Backbone manufacturer
    unknown
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cyclin B1
  • Species
    H. sapiens (human)
  • Mutation
    L45A
  • Entrez Gene
    CCNB1 (a.k.a. CCNB)
  • Promoter CMV
  • Tag / Fusion Protein
    • Venus (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVenus-N1 cyclin B1 L45A (1324) was a gift from Jonathon Pines (Addgene plasmid # 61851 ; http://n2t.net/addgene:61851 ; RRID:Addgene_61851)
  • For your References section:

    Co-activator independent differences in how the metaphase and anaphase APC/C recognise the same substrate. Matsusaka T, Enquist-Newman M, Morgan DO, Pines J. Biol Open. 2014 Sep 12;3(10):904-12. doi: 10.1242/bio.20149415. 10.1242/bio.20149415 PubMed 25217616