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Addgene

pIRES-mRFP-NLS-PCNA (2514)
(Plasmid #61850)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61850 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESneo
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PCNA
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_002592.2
  • Entrez Gene
    PCNA (a.k.a. ATLD2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on backbone)
    • mRFP (N terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMF-F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES-mRFP-NLS-PCNA (2514) was a gift from Jonathon Pines (Addgene plasmid # 61850 ; http://n2t.net/addgene:61850 ; RRID:Addgene_61850)
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