(R42A) Cyclin B1-GFP (62)
(Plasmid #61849)

• Purpose: mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable
• Depositing Lab: Jonathon Pines
• Publication: Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

Backbone

• Vector backbone: pCMX
• Backbone manufacturer: K.Umesono, Salk Institute
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: cyclin B1
• Species: H. sapiens (human)
• Mutation: V5A R42A
• Entrez Gene: CCNB1 (a.k.a. CCNB)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Myc (N terminal on insert)
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is an addition conservative mutation at position Val5 (Ala in this construct).