pcDNA5/FRT/TO(Neo)-Venus-Flag-WT wobbled cdc20 (1991)
(Plasmid
#61848)
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Purposefor making stable RPE cells expressing WT cdc20
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61848 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5/FRT/TO(Neo)-Venus-Flag
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Backbone manufacturerPines Lab
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecdc20
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SpeciesH. sapiens (human)
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Mutationwobbled
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Entrez GeneCDC20 (a.k.a. CDC20A, OOMD14, OZEMA14, bA276H19.3, p55CDC)
- Promoter CMV
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Tags
/ Fusion Proteins
- Flag (N terminal on backbone)
- Venus (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5/FRT/TO(Neo)-Venus-Flag-WT wobbled cdc20 (1991) was a gift from Jonathon Pines (Addgene plasmid # 61848 ; http://n2t.net/addgene:61848 ; RRID:Addgene_61848)