pcDNA5 FRT/TO/STRP 3x FLAG cyclin B1 L45A (1391)
(Plasmid
#61841)
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Purposemammalian expression of cyclin B1 non-degradable L45A mutant
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61841 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5/FRT/TO/STREP 3x FLAG
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Backbone manufacturerunknown
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecyclin B1
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SpeciesH. sapiens (human)
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MutationL45A
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Entrez GeneCCNB1 (a.k.a. CCNB)
- Promoter CMV
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Tags
/ Fusion Proteins
- 2xSTREP (C terminal on backbone)
- 3xFLAG (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5 FRT/TO/STRP 3x FLAG cyclin B1 L45A (1391) was a gift from Jonathon Pines (Addgene plasmid # 61841 ; http://n2t.net/addgene:61841 ; RRID:Addgene_61841) -
For your References section:
APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment. Mansfeld J, Collin P, Collins MO, Choudhary JS, Pines J. Nat Cell Biol. 2011 Sep 18;13(10):1234-43. doi: 10.1038/ncb2347. 10.1038/ncb2347 PubMed 21926987