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Purposemammalian expression of cdc2
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61840 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecdc2
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Alt nameCDK1
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SpeciesH. sapiens (human)
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Entrez GeneCDK1 (a.k.a. CDC2, CDC28A, P34CDC2)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this construct may have a few mismatches from the available sequence for the CDK1 UTR. These changes do not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-cdc2 (716) was a gift from Jonathon Pines (Addgene plasmid # 61840 ; http://n2t.net/addgene:61840 ; RRID:Addgene_61840) -
For your References section:
MPF localization is controlled by nuclear export. Hagting A, Karlsson C, Clute P, Jackman M, Pines J. EMBO J. 1998 Jul 15;17(14):4127-38. 10.1093/emboj/17.14.4127 PubMed 9670027