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pT7 cyclin A1-164 venus cks1 (2196)
(Plasmid #61839)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61839 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pt7 venus cks1 (1843)
  • Backbone manufacturer
    Pines Lab
  • Vector type
    in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cyclin A
  • Alt name
    csk1
  • Species
    H. sapiens (human)
  • Mutation
    L2V
  • Entrez Gene
    CCNA2 (a.k.a. CCN1, CCNA)
  • Promoter T7
  • Tag / Fusion Protein
    • Venus (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7 cyclin A1-164 venus cks1 (2196) was a gift from Jonathon Pines (Addgene plasmid # 61839 ; http://n2t.net/addgene:61839 ; RRID:Addgene_61839)

  • For your References section:

    How cyclin A destruction escapes the spindle assembly checkpoint. Di Fiore B, Pines J. J Cell Biol. 2010 Aug 23;190(4):501-9. 10.1083/jcb.201001083 PubMed 20733051
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