pGEX6P1-GFP-Nanobody
(Plasmid #61838)

• Purpose: Expresses GST-tagged anti-GFP Nanobody in E. coli cells.
• Depositing Lab: Kazuhisa Nakayama
• Publication: Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740.

Backbone

• Vector backbone: pGEX6P1
• Backbone manufacturer: GE healthcare
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 5320
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GFP-Nanobody
• Species: Synthetic
• Insert Size (bp): 355
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CGTATTGAAGCTATCCCAC
• 3′ sequencing primer: CGGCATCCGCTTACAGAC

Resource Information

• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry