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Addgene

pSABAD14-111
(Plasmid #61824)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61824 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 4597
  • Total vector size (bp) 5206
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NpuDnaB(delta283)dN11 intein
  • Alt name
    NpuDnaB(delta283)dN11 C-intein
  • Species
    Nostoc punctiforme
  • Insert Size (bp)
    609
  • Promoter pBAD
  • Tag / Fusion Protein
    • GB1-H6 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer pBAD Forward
  • 3′ sequencing primer pBAD Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSABAD14-111 was a gift from Hideo Iwai (Addgene plasmid # 61824 ; http://n2t.net/addgene:61824 ; RRID:Addgene_61824)

  • For your References section:

    Structure-based engineering and comparison of novel split inteins for protein ligation. Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. 10.1039/c4mb00021h PubMed 24574026
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