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Addgene

pSARSF53-455
(Plasmid #61819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61819 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3559
  • Total vector size (bp) 3937
  • Modifications to backbone
    deletion of MCS-2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PhoRadN38 intein
  • Alt name
    PhoRadN13 N-intein
  • Species
    Pyrococcus horikoshii
  • Insert Size (bp)
    378
  • Promoter T7
  • Tag / Fusion Protein
    • H6-Smt3 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSARSF53-455 was a gift from Hideo Iwai (Addgene plasmid # 61819 ; http://n2t.net/addgene:61819 ; RRID:Addgene_61819)

  • For your References section:

    Structure-based engineering and comparison of novel split inteins for protein ligation. Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. 10.1039/c4mb00021h PubMed 24574026
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