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Purpose(Empty Backbone) pUASp plasmid with attB for Drosophila transgenesis
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61814 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPMW
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Backbone manufacturerGateway
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Modifications to backboneinsertion of attB
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Vector typeInsect Expression
- Promoter UASp
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Tag
/ Fusion Protein
- 6xMyc (N terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pUASp-5
- 3′ sequencing primer pUASp-3 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPMW-attB was a gift from Frederique Peronnet (Addgene plasmid # 61814 ; http://n2t.net/addgene:61814 ; RRID:Addgene_61814) -
For your References section:
Cyclin G and the Polycomb Repressive Complexes PRC1 and PR-DUB cooperate for developmental stability. Dardalhon-Cumenal D, Deraze J, Dupont CA, Ribeiro V, Coleno-Costes A, Pouch J, Le Crom S, Thomassin H, Debat V, Randsholt NB, Peronnet F. PLoS Genet. 2018 Jul 11;14(7):e1007498. doi: 10.1371/journal.pgen.1007498. 10.1371/journal.pgen.1007498 PubMed 29995890
Map uploaded by the depositor.
