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pGL3-E-cadherin promoter
(Plasmid #61798)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61798 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    -178/+92 fragment of the mouse Cdh1 promoter
  • Alt name
    E-cadherin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    270
  • Entrez Gene
    Cdh1 (a.k.a. ARC-1, E-cad, Ecad, L-CAM, UVO, Um)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer 5´-CTAGCAAAATAGGCTGTCCC-3´
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-E-cadherin promoter was a gift from Judy Lieberman (Addgene plasmid # 61798 ; http://n2t.net/addgene:61798 ; RRID:Addgene_61798)

  • For your References section:

    miR-200 promotes the mesenchymal to epithelial transition by suppressing multiple members of the Zeb2 and Snail1 transcriptional repressor complexes. Perdigao-Henriques R, Petrocca F, Altschuler G, Thomas MP, Le MT, Tan SM, Hide W, Lieberman J. Oncogene. 2015 Mar 23. doi: 10.1038/onc.2015.69. 10.1038/onc.2015.69 PubMed 25798844
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