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psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT
(Plasmid #61789)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61789 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psiCHECK-2
  • Backbone size w/o insert (bp) 6273
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1001
  • Mutation
    both miR-200c MRE-2 and MRE-3 mutated

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer 5'-GTCCGCAACTACAACGCCTACCTT-3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT was a gift from Judy Lieberman (Addgene plasmid # 61789 ; http://n2t.net/addgene:61789 ; RRID:Addgene_61789)

  • For your References section:

    miR-200 promotes the mesenchymal to epithelial transition by suppressing multiple members of the Zeb2 and Snail1 transcriptional repressor complexes. Perdigao-Henriques R, Petrocca F, Altschuler G, Thomas MP, Le MT, Tan SM, Hide W, Lieberman J. Oncogene. 2015 Mar 23. doi: 10.1038/onc.2015.69. 10.1038/onc.2015.69 PubMed 25798844
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