psiCHECK-2-Smad5-3'UTR
(Plasmid
#61777)
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PurposeLuciferase reporter containing the 3'UTR of mouse Smad5
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61777 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepsiCHECK-2
- Backbone size w/o insert (bp) 6273
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemouse Smad5 3'UTR
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SpeciesM. musculus (mouse)
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Insert Size (bp)4900
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AsiSI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer 5'-GTCCGCAACTACAACGCCTACCTT-3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note that the UTR sequence in this plasmid may have some minor discrepancies with top GenBank hits
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
psiCHECK-2-Smad5-3'UTR was a gift from Judy Lieberman (Addgene plasmid # 61777 ; http://n2t.net/addgene:61777 ; RRID:Addgene_61777) -
For your References section:
miR-200 promotes the mesenchymal to epithelial transition by suppressing multiple members of the Zeb2 and Snail1 transcriptional repressor complexes. Perdigao-Henriques R, Petrocca F, Altschuler G, Thomas MP, Le MT, Tan SM, Hide W, Lieberman J. Oncogene. 2015 Mar 23. doi: 10.1038/onc.2015.69. 10.1038/onc.2015.69 PubMed 25798844