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Addgene

pEFIRES NQO1 Y128F
(Plasmid #61734)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61734 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEFIRES
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NAD(P)H dehydrogenase, quinone 1 (NQO1)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    825
  • Mutation
    Y128F
  • GenBank ID
    NM_000903.2 NP_000894.1
  • Entrez Gene
    NQO1 (a.k.a. DHQU, DIA4, DTD, NMOR1, NMORI, QR1)
  • Promoter Ef1a
  • Tag / Fusion Protein
    • None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer as for pEFIRES NQO1 WT
  • 3′ sequencing primer as for pEFIRES NQO1 WT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing results find that the Y to F mutation is at position 129 with respect to reference sequence NP_000894.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEFIRES NQO1 Y128F was a gift from Yosef Shaul (Addgene plasmid # 61734 ; http://n2t.net/addgene:61734 ; RRID:Addgene_61734)

  • For your References section:

    A mechanism of ubiquitin-independent proteasomal degradation of the tumor suppressors p53 and p73. Asher G, Tsvetkov P, Kahana C, Shaul Y. Genes Dev. 2005 Feb 1;19(3):316-21. 10.1101/gad.319905 PubMed 15687255
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