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Addgene

mCherry-Parkin-IRES-zeo
(Plasmid #61727)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61727 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LZRS
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    Slow growing. May need additional incubation time.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Parkin
  • Alt name
    Park 2
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_004562
  • Entrez Gene
    PRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer mCherry Forward
  • 3′ sequencing primer pCDH-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Dr. Richard Youle provided mCherry-Parkin (Addgene Plasmid 23956).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-Parkin-IRES-zeo was a gift from Douglas Green & Stephen Tait (Addgene plasmid # 61727 ; http://n2t.net/addgene:61727 ; RRID:Addgene_61727)

  • For your References section:

    Widespread mitochondrial depletion via mitophagy does not compromise necroptosis. Tait SW, Oberst A, Quarato G, Milasta S, Haller M, Wang R, Karvela M, Ichim G, Yatim N, Albert ML, Kidd G, Wakefield R, Frase S, Krautwald S, Linkermann A, Green DR. Cell Rep. 2013 Nov 27;5(4):878-85. doi: 10.1016/j.celrep.2013.10.034. Epub 2013 Nov 21. 10.1016/j.celrep.2013.10.034 PubMed 24268776
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