pINTO-NSA::mEzh2
(Plasmid #61725)

• Purpose: Expression of mouse Ezh2 fused to monomeric streptavidin (N-terminus)
• Depositing Lab: Roberto Bonasio
• Publication: Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-43. doi: 10.1021/pr500196b. Epub 2014 Oct 29.

Backbone

• Vector backbone: pINTO-N-SA
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Enhancer of zeste homolog 2
• Species: M. musculus (mouse)
• Insert Size (bp): 2241
• GenBank ID: NM_007971.2
• Entrez Gene: Ezh2 (a.k.a. Enx-1, Enx1h, KMT6, mKIAA4065)
• Tag / Fusion Protein:
  • mSA (monomeric streptavidin) (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CTC GTT TAG TGA ACC GTC AG
• 3′ sequencing primer: AGATGGCTGGCAACTAGAAG

Resource Information

• Supplemental Documents:
  • Annotated plasmid sequence (GeneBank format)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry