pRS415-Cox15-13xMyc
(Plasmid
#61721)
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Purposeyeast Cox15 heme A synthase with C-terminal 13xMyc tag in low-copy yeast expression vector
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61721 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS415
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Backbone manufacturerStratagene
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCox15
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneCOX15 (a.k.a. YER141W)
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Tag
/ Fusion Protein
- 13xMyc (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS415-Cox15-13xMyc was a gift from Oleh Khalimonchuk (Addgene plasmid # 61721 ; http://n2t.net/addgene:61721 ; RRID:Addgene_61721) -
For your References section:
Analysis of Oligomerization Properties of Heme a Synthase Provides Insights into its Function in Eukaryotes. Swenson S, Cannon A, Harris NJ, Taylor NG, Fox JL, Khalimonchuk O. J Biol Chem. 2016 Mar 3. pii: jbc.M115.707539. 10.1074/jbc.M115.707539 PubMed 26940873