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Addgene

pRS425-M25-Afg1
(Plasmid #61720)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61720 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS425
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 6849
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Afg1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    AFG1 (a.k.a. YEL052W)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS425-M25-Afg1 was a gift from Oleh Khalimonchuk (Addgene plasmid # 61720 ; http://n2t.net/addgene:61720 ; RRID:Addgene_61720)

  • For your References section:

    The AAA ATPase Afg1 preserves mitochondrial fidelity and cellular health by maintaining mitochondrial matrix proteostasis. Germany EM, Zahayko N, Huebsch ML, Fox JL, Prahlad V, Khalimonchuk O. J Cell Sci. 2018 Nov 21;131(22). pii: jcs.219956. doi: 10.1242/jcs.219956. 10.1242/jcs.219956 PubMed 30301782
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