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Purpose(Empty Backbone) Gateway compatible BiFC vector (pEarleyGate201-YN)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61704 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEarleyGate-201
- Backbone size (bp) 11778
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Modifications to backboneYFP-N was fused after the Gateway cassette
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Vector typePlant Expression ; BiFC assay
- Promoter 35S
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Selectable markersBasta
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Tag
/ Fusion Protein
- YFP-N and HA (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGTQL1211YN was a gift from Yuhai Cui (Addgene plasmid # 61704 ; http://n2t.net/addgene:61704 ; RRID:Addgene_61704) -
For your References section:
Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y. Plant J. 2010 Jan;61(2):259-70. doi: 10.1111/j.1365-313X.2009.04048.x. Epub 2009 Oct 16. 10.1111/j.1365-313X.2009.04048.x PubMed 19843313