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Addgene

pPd4E-ISH
(Plasmid #61700)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61700 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHellsGate12
  • Backbone size w/o insert (bp) 17680
  • Vector type
    RNAi
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PdeIF4E
  • Species
    Plum
  • Insert Size (bp)
    350
  • Promoter 35S

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CTTCGCAAGACCTTCCTCT
  • 3′ sequencing primer GTAAATGCTCCAGAACTCCTCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPd4E-ISH was a gift from Lining Tian (Addgene plasmid # 61700 ; http://n2t.net/addgene:61700 ; RRID:Addgene_61700)

  • For your References section:

    Silencing of the host factor eIF(iso)4E gene confers plum pox virus resistance in plum. Wang X, Kohalmi SE, Svircev A, Wang A, Sanfacon H, Tian L. PLoS One. 2013;8(1):e50627. doi: 10.1371/journal.pone.0050627. Epub 2013 Jan 28. PONE-D-12-26441 [pii] PubMed 23382802
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