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Addgene

pET15b-12x
(Plasmid #61699)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61699 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Opt12x
  • Species
    Pseudomonas stutzeri
  • Insert Size (bp)
    1014
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pBRrevBam; T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid is pET15b-12x and NOT the mutant plasmid pET15b-12x-A176R.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b-12x was a gift from Huimin Zhao (Addgene plasmid # 61699 ; http://n2t.net/addgene:61699 ; RRID:Addgene_61699)

  • For your References section:

    Efficient regeneration of NADPH using an engineered phosphite dehydrogenase. Johannes TW, Woodyer RD, Zhao H. Biotechnol Bioeng. 2007 Jan 1;96(1):18-26. 10.1002/bit.21168 PubMed 16948172
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