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Addgene

pET15b SARS-CoV PLpro
(Plasmid #61693)

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Item Catalog # Description Quantity Price (USD)
Plasmid 61693 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5708
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV PLpro
  • Species
    SARS
  • Promoter T7
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For protein expression, grow in Rosetta cells.
25 degree induction for 14-15 hours with 0.5 mM IPTG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b SARS-CoV PLpro was a gift from Michael Johnson (Addgene plasmid # 61693 ; http://n2t.net/addgene:61693 ; RRID:Addgene_61693)

  • For your References section:

    Synergistic inhibitor binding to the papain-like protease of human SARS coronavirus: mechanistic and inhibitor design implications. Lee H, Cao S, Hevener KE, Truong L, Gatuz JL, Patel K, Ghosh AK, Johnson ME. ChemMedChem. 2013 Aug;8(8):1361-72. doi: 10.1002/cmdc.201300134. Epub 2013 Jun 20. 10.1002/cmdc.201300134 PubMed 23788528
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