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Addgene

pGEX-6P-1 SARS-CoV 3CLpro
(Plasmid #61692)

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Item Catalog # Description Quantity Price (USD)
Plasmid 61692 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pGEX-6P-1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4984
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS 3CLpro protease
  • Species
    SARS
  • Promoter tac
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • HIS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For protein expression, grow in Rosetta cells.
25 degree induction for 14-15 hours with 0.5 mM IPTG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-6P-1 SARS-CoV 3CLpro was a gift from Michael Johnson (Addgene plasmid # 61692 ; http://n2t.net/addgene:61692 ; RRID:Addgene_61692)

  • For your References section:

    Identification of novel drug scaffolds for inhibition of SARS-CoV 3-Chymotrypsin-like protease using virtual and high-throughput screenings. Lee H, Mittal A, Patel K, Gatuz JL, Truong L, Torres J, Mulhearn DC, Johnson ME. Bioorg Med Chem. 2014 Jan 1;22(1):167-77. doi: 10.1016/j.bmc.2013.11.041. Epub 2013 Dec 1. 10.1016/j.bmc.2013.11.041 PubMed 24332657
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