pNIC28-Bsa4-TF
(Plasmid
#61689)
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Purpose(Empty Backbone) E. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with the TEE and trigger factor sequences inserted
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61689 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNIC28-Bsa4 (addgene #26103)
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Backbone manufacturerOpher Gileadi
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Modifications to backbonea translation enhancing element (TEE) and Trigger Factor (TF) chaperone sequences inserted (based on pCold-TF from Clontech)
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Vector typeBacterial Expression
- Promoter T7
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Tags
/ Fusion Proteins
- HIS (N terminal on backbone)
- Trigger Factor (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNIC28-Bsa4-TF was a gift from Brian Smith (Addgene plasmid # 61689 ; http://n2t.net/addgene:61689 ; RRID:Addgene_61689)