pET28-Rsn2
(Plasmid
#61686)
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Purposebacterial expression of ranaspumin 2 as a His-tagged fusion protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61686 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-28
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5369
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRanaspumin-2
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Alt nameRsn-2
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Speciestúngara frog (Engystomops pustulosus)
- Promoter T7
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28-Rsn2 was a gift from Brian Smith (Addgene plasmid # 61686 ; http://n2t.net/addgene:61686 ; RRID:Addgene_61686) -
For your References section:
Ranaspumin-2: structure and function of a surfactant protein from the foam nests of a tropical frog. Mackenzie CD, Smith BO, Meister A, Blume A, Zhao X, Lu JR, Kennedy MW, Cooper A. Biophys J. 2009 Jun 17;96(12):4984-92. doi: 10.1016/j.bpj.2009.03.044. 10.1016/j.bpj.2009.03.044 PubMed 19527658
