pET32-sLatherin
(Plasmid
#61685)
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Purposebacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusion
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61685 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-32
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLatherin
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Specieshorse
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MutationCodon optimized for E.coli
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Entrez GeneLATH
- Promoter T7
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Tags
/ Fusion Proteins
- Trx-Tag thioredoxin protein (N terminal on backbone)
- HIS (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET32-sLatherin was a gift from Brian Smith (Addgene plasmid # 61685 ; http://n2t.net/addgene:61685 ; RRID:Addgene_61685) -
For your References section:
The structure of latherin, a surfactant allergen protein from horse sweat and saliva. Vance SJ, McDonald RE, Cooper A, Smith BO, Kennedy MW. J R Soc Interface. 2013 Jun 19;10(85):20130453. doi: 10.1098/rsif.2013.0453. Print 2013 Aug 6. 10.1098/rsif.2013.0453 PubMed 23782536