HIV-1 integrase W235F in pET-15b
(Plasmid
#61681)
-
PurposeHIV-1 integrase W235F in pET-15b
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61681 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET-15b
- Backbone size w/o insert (bp) 5708
- Total vector size (bp) 5708
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameHIV-1 integrase W235F
-
Alt nameHIV-1 IN W235F
-
SpeciesSynthetic
-
Insert Size (bp)900
-
MutationW235F
- Promoter T7
-
Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nde I (unknown if destroyed)
- 3′ cloning site BamH I (unknown if destroyed)
- 5′ sequencing primer T7 promotor
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
HIV-1 integrase W235F in pET-15b was a gift from Robert Craigie (Addgene plasmid # 61681 ; http://n2t.net/addgene:61681 ; RRID:Addgene_61681) -
For your References section:
Retroviral DNA integration: reaction pathway and critical intermediates. Li M, Mizuuchi M, Burke TR Jr, Craigie R. EMBO J. 2006 Mar 22;25(6):1295-304. Epub 2006 Feb 16. 10.1038/sj.emboj.7601005 PubMed 16482214