HIV-1 integrase F185H/C280S in pET15b
(Plasmid
#61672)
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PurposeHIV-1 integrase F185H/C280S in pET-15b
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61672 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-15b
- Backbone size w/o insert (bp) 5708
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHIV-1 integrase F185H/C280S
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Alt nameHIV-1 IN F185H/C280S
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SpeciesSynthetic
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Insert Size (bp)900
- Promoter T7 promotor
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nde I (not destroyed)
- 3′ cloning site BamH I (not destroyed)
- 5′ sequencing primer T7 promotor
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HIV-1 integrase F185H/C280S in pET15b was a gift from Robert Craigie (Addgene plasmid # 61672 ; http://n2t.net/addgene:61672 ; RRID:Addgene_61672) -
For your References section:
A soluble active mutant of HIV-1 integrase: involvement of both the core and carboxyl-terminal domains in multimerization. Jenkins TM, Engelman A, Ghirlando R, Craigie R. J Biol Chem. 1996 Mar 29;271(13):7712-8. PubMed 8631811